Difference between revisions of "Rhodamine B"

Latest revision as of 12:12, 20 September 2010

Rhodamine B
File:Rhodamine B.svg
IUPAC name [9-(2-carboxyphenyl)-6-diethylamino-3-xanthenylidene]-diethylammonium chloride
Other names Rhodamine 610, C.I. Pigment Violet 1, Basic Violet 10, C.I. 45170
style="background: #F8EABA; text-align: center;" colspan="2" | Identifiers
CAS number	81-88-9 Y
PubChem	6694
SMILES	Script error: No such module "collapsible list".
style="background: #F8EABA; text-align: center;" colspan="2" | Properties
Molecular formula	C28H31ClN2O3
Molar mass	479.02
Appearance	red to violet powder
Melting point	210 - 211 °C (Decomposes)
Solubility in water	~50 g/l
style="background: #F8EABA; text-align: center;" colspan="2" | Hazards
MSDS	Sigma
Y (what is this?)  (verify) Except where noted otherwise, data are given for materials in their standard state (at 25 °C, 100 kPa)
Infobox references

Rhodamine B (pronounced /ˈroʊdəmiːn/) is a chemical compound and a dye. It is often used as a tracer dye within water to determine the rate and direction of flow and transport. Rhodamine dyes fluoresce and can thus be detected easily and inexpensively with instruments called fluorometers. Rhodamine dyes are used extensively in biotechnology applications such as fluorescence microscopy, flow cytometry, fluorescence correlation spectroscopy and ELISA.

Rhodamine B is used in biology as a staining fluorescent dye, sometimes in combination with auramine O, as the auramine-rhodamine stain to demonstrate acid-fast organisms, notably Mycobacterium.

Rhodamine B is tunable around 610 nm when used as a laser dye ^[1]. Its luminescence quantum yield is 0.65 in basic ethanol^[2], 0.49 in ethanol ^[3], 1.0 ^[4], and 0.68 in 94% ethanol ^[5]. The fluorescence yield is temperature dependent ^[6].

Solubility

The solubility in water is ~50 g/l. However, the solubility in acetic acid solution (30 vol.%) is ~400 g/l. Chlorinated tap water decomposes rhodamine B. Rhodamine B solutions adsorb to plastics and should be kept in glass ^[7].

Other uses

Rhodamine B is being tested for use as a biomarker in oral rabies vaccines for wildlife, such as raccoons, to identify animals that have eaten a vaccine bait. The rhodamine is incorporated into the animal's whiskers and teeth. ^[8]

It is also often mixed with herbicides to show where they have been used.

Safety and health

In California, Rhodamine B is suspected to be carcinogenic and thus products containing it must contain a warning on its label.^[9]

In New Jersey, MSDS files state that there is limited evidence of carcinogenicity in laboratory animal, and no evidence at all in humans. ^[10]

References

See also

• Dye laser
• Rhodamine
• Rhodamine 6G

it:Rodamina B

1. ↑ Rhodamine B
2. ↑ R. F. Kubin and A. N. Fletcher, "Fluorescence quantum yields of some rhodamine dyes." J. Luminescence 27 (1982) 455
3. ↑ K. G. Casey and E. L. Quitevis, "Effect of solvent polarity on nonradiative processes in xanthene dyes: Rhodamine B in normal alcohols," J. Phys. Chem., 92, 6590-6594, 1988
4. ↑ R. E. Kellogg and R. G. Bennett, "Radiationless intermolecular energy transfer. III. Determination of phosphorescence efficiencies.," J. Chem. Phys., 41, 3042-3045, 1964
5. ↑ M. J. Snare, F. E. Treloar, K. P. Ghiggino, and P. J. Thistlethwaite "The photophysics of rhodamine B.," J. Photochem., 18, 335-346, 1982
6. ↑ T. Karstens and K. Kobs, "Rhodamine B and Rhodamine 101 as reference substances for fluorescence quantum yield measurements." J. Phys. Chem., 84, 1871-1872, 1980
7. ↑ "Detection and prevention of leaks from dams" By Antonio Plata Bedmar and Luís Araguás Araguás, Taylor & Francis, 2002, ISBN 9058093557, 9789058093554
8. ↑ Oral Rabies Vaccination in North America: Opportunities, Complexities, and Challenges
9. ↑ http://www.brown.edu/Departments/Visual_Art/documents/NavalJelly.pdf, Naval Jelly msds with Rhodamine B
10. ↑ http://www.jtbaker.com/msds/englishhtml/r5400.htm, J.T.Baker Rhodamine B MSDS