Difference between revisions of "Chelex 100"
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Latest revision as of 19:16, 21 September 2010
Chelex 100 is a chelating material from Bio-Rad used to purify other compounds via ion exchange. It is noteworthy for its ability to bind transition metal ions.
It is a styrene-divinylbenzene co-polymer containing iminodiacetic acid groups.
A concentrated solution of metals is obtained by eluting the resin with a small volume of 2 M nitric acid, which protonates the iminodiacetate groups.
Chelex resin is often used for DNA extraction. Polar resin beads bind polar cellular components after breaking open cells. Non-polar nuclear DNA and RNA remain in water solution above chelex.
References
- Daniel Harris. Quantitative Chemical Analysis, seventh edition, 2007. ISBN 0-7167-7041-5. Page 594.
- R. N. Ceo, M. R. Kazerouni, and K. Rengan (1993). "Sorption of silver ions by Chelex 100 chelating resin". Journal of Radioanalytical and Nuclear Chemistry, Articles. 172: 43–48. doi:10.1007/BF02040660.
External links
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