|style="background: #F8EABA; text-align: center;" colspan="2" | Identifiers|
|CAS number||, |
|RTECS number|| DV8750000|
|style="background: #F8EABA; text-align: center;" colspan="2" | Properties|
|Molecular formula|| C12H14N4|
|Molar mass|| 214.27 g/mol|
360.11 g/mol (4HCl)
|style="background: #F8EABA; text-align: center;" colspan="2" | Hazards|
|EU classification||Harmful (Xn)|
|LD50||mouse, oral Acute: 1834 mg/kg.|
|Except where noted otherwise, data are given for materials in their standard state (at 25 °C, 100 kPa)|
3,3'-Diaminobenzidine (DAB) is an organic compound. This derivative of benzidine is a precursor to polybenzimidazole fiber. As its water-soluble tetrahydrochloride, it has been used in immunohistochemical staining of nucleic acids and proteins.
Diaminobenzidine is oxidized by hydrogen peroxide in the presence of hemoglobin to give a dark brown color. This is used to detect fingerprints in blood. Improperly prepared tissue samples may give false positives In neuroscience, this reaction is used to stain neurons which were prepared to contain hydrogen peroxidase, using complexes of injected biocytin with avidine or streptavidine, biotin, and the peroxidase.
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