Carnoy's solution

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Carnoy's Solution is a fixative composed of 60% ethanol, 30% chloroform and 10% glacial acetic acid.[1]

In Oral and Maxillofacial Pathology: A Rationale for Diagnosis and Treatment[2], the authors state that Carnoy's Solution is composed of:

This is the same proportion by volume as the previous formula, with the addition of Ferric Chloride.

Uses

Some of the uses of Carnoy's solution are:

  • Enhancing lymph node detection during dissection of cadavers.[3]
  • Immunohistochemical fixation and detection of NMDA receptors within the murine hippocampus.[4]
  • Applied directly following enucleation for the treatment of keratocystic odontogenic tumors.[5][6][7]
  • In some cases direct application following enucleation for certain kinds of unicystic ameloblastomas.[8] This appears to decrease the likelihood of recurrence over enucleation alone.[9] Protein coagulation is thought to limit uptake of these toxic materials by surrounding tissues, however it is this fact that limits its usefulness as a treatment agent in general.[2]
  • As a fixative for pap smear samples.[10]
  • As a fixative agent for both nuclear and mitochondrial DNA in various tissues.[11]
  • As a fixative agent to preserve mucus, useful for tissue preparation before staining with periodic acid-Schiff base.[12]

References

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  1. "MSDS :: Carnoy's Solution (Fixative)". Retrieved 13 JAN 2009.  Check date values in: |access-date= (help)
  2. 2.0 2.1 Marx, Robert E.; Stern, Diane (2003). Oral and maxillofacial pathology: a rationale for diagnosis and treatment. Chicago: Quintessence. p. 684. ISBN 0-86715-390-3. 
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  6. "Odontogenic Keratocyst: The Northwestern USA Experience". Retrieved 14 JAN 2009.  Check date values in: |access-date= (help)
  7. "Use of Carnoy's Solution in management of odontogenic keratocysts". Retrieved 14 JAN 2009.  Check date values in: |access-date= (help)
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  10. Shamsi M, Abdali K, Montazer NR, Kumar PV, Tabatabaee HR (2008). "Comparison of Carnoy's solution and 96% ethyl alcohol fixation in bloody Pap smears". Acta Cytol. 52 (2): 187–90. PMID 18499991. 
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  12. "Stains File: Carnoy's Fluid". Retrieved 25. OCT 2009.  Check date values in: |access-date= (help)