Indo-1
From Self-sufficiency
Indo-1 | |
---|---|
File:Indo-1.svg | |
2-[4-(bis(carboxymethyl)amino)-3-[2-[2-(bis(carboxymethyl)amino)-
5-methylphenoxy]ethoxy]phenyl]-1H-indole-6-carboxylic acid | |
Other names Indo-1 | |
style="background: #F8EABA; text-align: center;" colspan="2" | Identifiers | |
CAS number | 96314-96-4 |
PubChem | 105060 |
SMILES | Script error: No such module "collapsible list". |
style="background: #F8EABA; text-align: center;" colspan="2" | Properties | |
Molecular formula | C32H31N3O12 |
Molar mass | 649.60 g/mol |
Except where noted otherwise, data are given for materials in their standard state (at 25 °C, 100 kPa) | |
Infobox references |
Indo-1 is a popular calcium indicator similar to Fura-2. In contrast to Fura-2, Indo-1 has a dual emissions peak. The main emission peak in calcium-free solution is 475 nm while in the presence of calcium the emission is shifted to 400 nm. It is widely used in flow cytometry. The penta potassium salt is commercially available and preferred to the free acid because of its higher solubility in water. While Indo-1 is not cell permeable the penta acetoxymethyl ester Indo-1 AM enters the cell where it is cleaved by intracellular esterases to Indo-1. The synthesis and properties of Indo-1 were presented in 1985 by the group of Roger Y Tsien.[1]
See also
23px | This section is empty. You can help by adding to it. |
External links
References
- ↑ Grynkiewicz G, Poenie M, Tsien RY (1985). "A new generation of Ca2+ indicators with greatly improved fluorescence properties". J. Biol. Chem. 260 (6): 3440–50. PMID 3838314.
40px | This biochemistry article is a stub. You can help ssf by expanding it. |
Categories:
- Pages with script errors
- CS1 maint: Multiple names: authors list
- Pages with broken file links
- 2Fix
- Articles containing unverified chemical infoboxes
- Articles using small message boxes
- Articles to be expanded from July 2010
- Articles with invalid date parameter in template
- All articles to be expanded
- Biochemistry methods
- Cell imaging
- Chelating agents
- Fluorescent dyes
- Biochemistry stubs