Nitrilotriacetic acid
| Nitrilotriacetic acid | |
|---|---|
| Skeletal formula of nitrilotriacetic acid | |
| 2,2',2''-nitrilotriacetic acid | |
| style="background: #F8EABA; text-align: center;" colspan="2" | Identifiers | |
| CAS number | 139-13-9 
|
| PubChem | 8758 |
| SMILES | Script error: No such module "collapsible list". |
| style="background: #F8EABA; text-align: center;" colspan="2" | Properties | |
| Molecular formula | C6H9NO6 |
| Molar mass | 191.14 g mol−1 |
 (what is this?) (verify)Except where noted otherwise, data are given for materials in their standard state (at 25 °C, 100 kPa) | |
| Infobox references | |
Nitrilotriacetic acid (NTA), C6H9NO6, is a polyamino carboxylic acid and is used as a chelating agent which forms coordination compounds with metal ions (chelates) such as Ca2+, Cu2+ or Fe3+.[1]
The uses of NTA are similar to that of EDTA. However, in contrast to EDTA, NTA is easily biodegradable and is almost completely removed during wastewater treatment.
In biotechnology applications it is used for protein isolation and purification. An aminobutanylated variant of NTA, called bis(carboxylmethyl)lysine, is linked to sephadex, which is used as a gel for affinity column chromatography. Because the nucleophilic nitrogen of imidazole (i.e. the Histidine side chain) can coordinate nickel cations, recombinant proteins containing a His-tag — 6 histidine residues at the C-terminus — will bind to the gel, while other proteins will not. After extensive washing to remove unbound protein, the tagged proteins are released by competition with a high concentration of imidazole.[2]
References
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<references />, or <references group="..." />de:Nitrilotriessigsäure fr:Acide nitrilotriacétique nl:Nitrilotriazijnzuur pl:Kwas nitrylotrioctowy
pt:Ácido nitrilotriacético- ↑ NITRILOTRIACETIC ACID AND ITS SALTS, International Agency for Research on Cancer (IARC)
- ↑ qiaexpressionist
