Difference between revisions of "Fluo-4"
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Latest revision as of 19:16, 21 September 2010
| Fluo-4 | |
|---|---|
| Fluo-4 Tetraanion | |
| Fluo-4 | |
| 2-{[2-(2-{5-[bis(carboxymethyl)amino]-2-methylphenoxy}ethoxy)-4-(2,7-difluoro-6-hydroxy-3-oxo-3H-xanthen-9-yl)phenyl](carboxymethyl)amino}acetic acid | |
| style="background: #F8EABA; text-align: center;" colspan="2" | Identifiers | |
| MeSH | Fluo-4 |
| ChEBI | 51103 |
| SMILES | Script error: No such module "collapsible list". |
| InChI | Script error: No such module "collapsible list". |
| Except where noted otherwise, data are given for materials in their standard state (at 25 °C, 100 kPa) | |
| Infobox references | |
Fluo-4 is used to measure calcium (Ca2+) concentrations inside living cells, and is often used for high-throughput screening of receptor ligands and calcium permeable ion channels.
The green-fluorescent calcium indicator, Fluo-4, is an improved version of the calcium indicator, Fluo-3. It is commonly used as the non-fluorescent acetoxymethyl ester (Fluo-4 AM) which is cleaved inside the cell to give the free, fluorescent Fluo-4. It loads faster, is brighter at equivalent concentrations and is well-excited by the 488 nm line of the argon-ion laser which is often used in biological research laboratories.[1] The sole distributor and owner of the patents is Invitrogen which acquired the original inventor Molecular Probes.
References
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