2,4-Dinitrochlorobenzene

2,4-Dinitrochlorobenzene
File:1-Chloro-2,4-dinitrobenzene.png
IUPAC name 1-Chloro-2,4-dinitro-benzene
Other names Dinitrochlorobenzene; Chlorodinitrobenzene; 2,4-Dinitrochlorobenzene; 2,4-Dinitrophenyl chloride; 4-Chloro-1,3-dinitrobenzene
style="background: #F8EABA; text-align: center;" colspan="2" | Identifiers
Abbreviations	CDNB; DNCB
CAS number	97-00-7
PubChem	6
ChemSpider	5
SMILES
style="background: #F8EABA; text-align: center;" colspan="2" | Properties
Molecular formula	C6H3ClN2O4
Molar mass	202.55 g mol−1
Except where noted otherwise, data are given for materials in their standard state (at 25 °C, 100 kPa)
Infobox references

2,4-Dinitrochlorobenzene (DNCB) is a benzene derivative. It is an electrophilic, cytotoxic compound that is used in biochemical research involving glutathione-S-transferases (GSTs).

Uses

DNCB induces a type IV hypersensitivity reaction in almost all people exposed to it, so it is used medically to assess the T cell activity in patients. This is a useful diagnostic test for immunocompromised patients. It can also be used to treat warts.

DNCB is used as a substrate in GST enzyme activity assays. The molecule is conjugated to a single molecule of reduced glutathione which then fluoresces at 340 nm. Affinity of CDNB for each class of GST varies and so it is not a good measure of activity for some forms (e.g. GST-T).

Safety

DNCB can cause contact dermatitis.