Carnoy's solution
From Self-sufficiency
Carnoy's Solution is a fixative composed of 60% ethanol, 30% chloroform and 10% glacial acetic acid.[1]
In Oral and Maxillofacial Pathology: A Rationale for Diagnosis and Treatment[2], the authors state that Carnoy's Solution is composed of:
- 1 g of Ferric Chloride (FeCl3) dissolved in 24 mL of absolute alcohol
- 12 mL of chloroform
- 4 mL of glacial acetic acid
This is the same proportion by volume as the previous formula, with the addition of Ferric Chloride.
Uses
Some of the uses of Carnoy's solution are:
- Enhancing lymph node detection during dissection of cadavers.[3]
- Immunohistochemical fixation and detection of NMDA receptors within the murine hippocampus.[4]
- Applied directly following enucleation for the treatment of keratocystic odontogenic tumors.[5][6][7]
- In some cases direct application following enucleation for certain kinds of unicystic ameloblastomas.[8] This appears to decrease the likelihood of recurrence over enucleation alone.[9] Protein coagulation is thought to limit uptake of these toxic materials by surrounding tissues, however it is this fact that limits its usefulness as a treatment agent in general.[2]
- As a fixative for pap smear samples.[10]
- As a fixative agent for both nuclear and mitochondrial DNA in various tissues.[11]
- As a fixative agent to preserve mucus, useful for tissue preparation before staining with periodic acid-Schiff base.[12]
References
Cite error: Invalid <references> tag;
parameter "group" is allowed only.
<references />, or <references group="..." />- ↑ "MSDS :: Carnoy's Solution (Fixative)". Retrieved 13 JAN 2009. Check date values in:
|access-date=(help) - ↑ 2.0 2.1 Marx, Robert E.; Stern, Diane (2003). Oral and maxillofacial pathology: a rationale for diagnosis and treatment. Chicago: Quintessence. p. 684. ISBN 0-86715-390-3.
- ↑ Lua error in package.lua at line 80: module 'Module:Citation/CS1/Suggestions' not found.
- ↑ Lua error in package.lua at line 80: module 'Module:Citation/CS1/Suggestions' not found.
- ↑ Lua error in package.lua at line 80: module 'Module:Citation/CS1/Suggestions' not found.
- ↑ "Odontogenic Keratocyst: The Northwestern USA Experience". Retrieved 14 JAN 2009. Check date values in:
|access-date=(help) - ↑ "Use of Carnoy's Solution in management of odontogenic keratocysts". Retrieved 14 JAN 2009. Check date values in:
|access-date=(help) - ↑ Lua error in package.lua at line 80: module 'Module:Citation/CS1/Suggestions' not found.
- ↑ Lua error in package.lua at line 80: module 'Module:Citation/CS1/Suggestions' not found.
- ↑ Shamsi M, Abdali K, Montazer NR, Kumar PV, Tabatabaee HR (2008). "Comparison of Carnoy's solution and 96% ethyl alcohol fixation in bloody Pap smears". Acta Cytol. 52 (2): 187–90. PMID 18499991.
- ↑ Lua error in package.lua at line 80: module 'Module:Citation/CS1/Suggestions' not found.
- ↑ "Stains File: Carnoy's Fluid". Retrieved 25. OCT 2009. Check date values in:
|access-date=(help)
