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Chemical structure of PIPES
style="background: #F8EABA; text-align: center;" colspan="2" | Identifiers
CAS number 5625-37-6
style="background: #F8EABA; text-align: center;" colspan="2" | Properties
Molecular formula C8H18N2O6S2
Molar mass 302.37
Appearance White powder
Melting point

Decomposes above 300 °C

Boiling point


Solubility in water 1 g/L (100 °C)
style="background: #F8EABA; text-align: center;" colspan="2" | Hazards
MSDS External MSDS
Main hazards Irritant
NFPA 704
Except where noted otherwise, data are given for materials in their standard state (at 25 °C, 100 kPa)
Infobox references

PIPES is the common name for piperazine-N,N′-bis(2-ethanesulfonic acid), a frequently used buffering agent in biochemistry. It is an ethanesulfonic acid buffer developed by Good et al. in the 1960s.[1]


PIPES has pKa near the physiological pH which makes it useful in cell culture work. PIPES has been documented minimizing lipid loss when buffering glutaraldehyde histology in plant and animal tissues.[2][3] Fungal zoospore fixation for fluorescence microscopy and electron microscopy were optimized with a combination of glutaraldehyde and formaldehyde in PIPES buffer.[4] It has a negligible capacity to bind divalent ions.


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See also

  1. Good, Norman E.; Winget, G. Douglas; Winter, Wilhelmina; Connolly, Thomas N.; Izawa, Seikichi; Singh, Raizada M. M. (1966). "Hydrogen Ion Buffers for Biological Research". Biochemistry. 5 (2): 467. doi:10.1021/bi00866a011. PMID 5942950. 
  2. Salema, R. and Brando, I., J. Submicr. Cytol., 9, 79 (1973).
  3. Schiff, R.I. and Gennaro, J.F., Scaning Electron Microsc., 3, 449 (1979).
  4. Hardham, A.R. (1985). "Studies on the cell surface of zoospores and cysts of the fungus Phytophthora cinnamomi: The influence of fixation on patterns of lectin binding". Journal of Histochemistry. 33 (2): 110. PMID 3918095.